Cell Line:                              ZR-75-1

Cell Description:                 Human ductal carcinoma; mammary gland epithelium primary; breast;  ascites (metastasis)

 

Culture Medium:                      RPMI 1640 medium with 4.5 g/L glucose, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 10 mM HEPES and 10% FBS

Subculturing:                          Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:4 to 1:6 is recommended

Fluid Renewal:                        2 to 3 times per week

Doubling Time:            80 hrs

Morphology:                            epithelial

Growth Properties:                 adherent

Tumorigenic:                           yes, forms tumors in nude mice

Receptors Expressed:           estrogen; steroid Related Products: purified DNA (ATCC 45530 purified DNA 45531)

Isoenzymes:                           G6PD, B

Products:                                mucin (apomucin, MUC-1, MUC-2)

Depositor:                               Engel Age / St

Description:                            The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.

Comments:                              established from a 63-year-old caucasian female

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ECACC number:                      87012601

ATCC designation:                  CRL 1500