Cell Line: WI 38 VA-13 subline 2RA
Cell Description: Human foetal lung
Culture medium: Minimum essential medium (Eagle) with Hanks' BSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 0,1mM non-essential amino acids and 10% fetal bovine serum
(optional: DMEM with 4,5 g/l glucose)
Karyotype: 2n =46; diploid except at high passage number
Comments: From normal embryonic lung of female. Have broadest human virus spectra, useful for isolation of rhinoviruses.
Description: This cell line is a SV40-transformed variant of the Wi38 cell line.
Virus Susceptibility: herpes simplex; vesicular stomatitis (Indiana); poliovirus 2
Biosafety Level: 2 (contain Papovavirus)
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
ECACC number: 85062512
ATCC designation: CCL 75.1
Reference: Exp. Cell Res. 1961; 25:585; Am. J. Hyg. 1962; 75:240