Cell Line:                              WI 38 VA-13 subline 2RA

Cell Description:                 Human foetal lung

 

Culture medium:                      Minimum essential medium (Eagle) with Hanks' BSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 0,1mM non-essential amino acids and 10% fetal bovine serum

(optional: DMEM with 4,5 g/l glucose)

Subculture routine:                 Split confluent cultures 1:3 to 1:6 i. e. seeding at 2-4 x 104 cells/cm2 using trypsin EDTA; incubate at 5% CO2, 37¡C.

Morphology:                            Fibroblast

Karyotype:                              2n =46; diploid except at high passage number

Comments:                              From normal embryonic lung of female. Have broadest human virus spectra, useful for isolation of rhinoviruses.

Description:                            This cell line is a SV40-transformed variant of the Wi38 cell line.

Virus Susceptibility:                herpes simplex; vesicular stomatitis (Indiana); poliovirus 2

Biosafety Level:                  2 (contain Papovavirus)

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

ECACC number:                      85062512

ATCC designation:                  CCL 75.1

Reference:                             Exp. Cell Res. 1961; 25:585; Am. J. Hyg. 1962; 75:240