Cell Line: Vero

Cell Description: Monkey kidney
Culture medium: DMEM + 10% FBS
Subculture routine: Split confluent cultures 1:3 to 1:6 i.e .seeding at 1 - 3 x 10.000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% C02; 37C.
Morphology: Fibroblast
Karyotype: 2n = 60; modal No 58
Research council deposit: No
Depositor: Dr. B .Thornton, PHLS CAMR, Porton Down, Salisbury

Nippon Rinsho 1963;21:1209

Comments: Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.
Applications: Virus studies: Polio, rubella, arboviruses and reoviruses.

ECACC Number: 84113001
ATCC designation: CCL 81

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