Cell Line:                              U-937

Cell Description:                 human lymphoma, histiocytic; macrophage


Culture Medium:                      RPMI 1640 medium with 4.5 g/L glucose, sodium bicarbonate, 2mM L-glutamine, 1,0 mM sodium pyruvate + 10% fetal bovine serum (optional with 10 mM HEPES)

Subculturing:                          Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 0.1 to 1 X 105 viable cells/ml, and maintain between 5 x 105 and 1 X 106 cells/ml.

Fluid Renewal:                        add fresh medium every 3 to 4 days

Morphology:                            monocyte

Growth Properties:                 suspension

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                       1

Receptors Expressed:           immunoglobulin (Fc); complement (C3)

Products:                                lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)

Depositor:                               Koren

Comments:                              established from a 37-year-old caucasian male

ECACC number:                      87010802

ATCC designation:                  CRL-1593.2


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