Cell Line:                              U-87 MG

Cell Description:                 human glioblastoma; astrocytoma; brain

 

Culture Medium:                      MEM Eagle with Earle's BSS, sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate, 0.1 mM non-essential amino acids + 10% FBS

Subculturing:                          Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Morphology:                            epithelial

Growth Properties:                 monolayer

Split Ratio:                               A ratio of 1:2 to 1:5 is recommended

Fluid Renewal:                        2 to 3 times weekly

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Tumorigenic:                           yes, in nude mice

Karyotype:                              Chromosome Frequency Distribution 50 Cells: 2n = 46. The stemline chromosome number is hypodiploid, the 2S component occurring at 5.4%. The following nine markers were common to most S metaphases: t(1q;?3), t(1pl22q), t(6p;?11q-), (6q;?7p), t(7q;?), del(12q), t(20;1p;9q), t(?8p;?), and M1. Neither homogeneously staining regions (HSR) nor double minutes (DM) were detected; all S metaphases were monosomic for the X chromosome.

Antigen Expression:               Blood Type A, Rh+

Isoenzymes:                           Me-2, 1; PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0017

Depositor:                               Ponten

Description:                            This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-15, ATCC HTB-16 and ATCC HTB-17) by J. Ponten and associates from 1966 to 1969.

Comments:                              established from a 44-year-old caucasian female, grade III

ATCC No.:                               HTB-14

References:

Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Cancer Res. 52: 2533-2529, 1992. Ponten J and Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. Li YM et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996.