Cell Line:                              SW-1353

Cell Description:                 human chondrosarcoma; bone


Culture Medium:                      Leibovitz's L-15 medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; FBS, 10%

Subculturing:                          Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks.

Morphology:                            fibroblast

Growth Properties:                 monolayer

Split Ratio:                               A ratio of 1:2 is recommended

Fluid Renewal:                        2 to 3 times weekly

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Karyotype:                              hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident

Antigen Expression:               Blood type O -

Isoenzymes:                           G6PD, B; PGM1, 1; PGM3, 2; ES-D, 2; AK-1, 1; GLO-1, 2 Phenotype Frequency Prod:   0.00009

Depositor:                               Leibovitz

Comments:                              established from a 72-year-old caucasian female; humerus

Description:                            The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.

ATCC No.:                               HTB-94


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