Cell Description: human chondrosarcoma; bone
Culture Medium: Leibovitz's L-15 medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; FBS, 10%
Subculturing: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks.
Growth Properties: monolayer
Split Ratio: A ratio of 1:2 is recommended
Fluid Renewal: 2 to 3 times weekly
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
Karyotype: hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident
Antigen Expression: Blood type O -
Isoenzymes: G6PD, B; PGM1, 1; PGM3, 2; ES-D, 2; AK-1, 1; GLO-1, 2 Phenotype Frequency Prod: 0.00009
Comments: established from a 72-year-old caucasian female; humerus
Description: The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.
ATCC No.: HTB-94
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