Cell Description: Human adenocarcinoma, colorectal; colon
Culture Medium: LeibowitzÕs L-15 medium with sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate + 10% FBS
Subculturing: Remove medium, rinse the culture twice with fresh 0.025% trypsin, 0.02% EDTA, add fresh trypsin, EDTA and incubate for 10 to 20 minutes at 37¡C. Add fresh medium, resuspend and centrifuge. Resuspend in fresh medium and dispense into new flasks.
Split Ratio: A ratio of 1:3 to 1:6 is recommended
Growth Properties: monolayer
Fluid Renewal: 1 to 2 times weekly
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
Tumorigenic: yes, in nude mice
Antigen Expression: blood type O; Rh+
Isoenzymes: G6PD, B; PGM1, 1; PGM3, 1-2; 6PGD, A; ES-D, 1;
Reverse Transcriptase: negative
Products: carcinoembryonic antigen (CEA) 2654 ng/106 cells/10 days; keratin
Oncogene: myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -
Depositor: A. Leibovitz
Description: Isolated from colorectal adenocarcinoma. The cells are CSAp negative (CSAp-) and colon antigen 3, negative. They are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes. N-myc and N-ras expression were not detected. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Comments: establised from a 73-year-old, grade III, Dukes« type A, caucasian male
Wright WC et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. Leibovitz A et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. Keesee SK et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. Hao L. et al. PC-407 inhibited proliferation and induced apoptosis in human colon cancer SW-1116 cells. Acta Pharmacol Sin 25(11): 1509-1514, 2004.