Cell Line:                              SK-OV-3

Cell Description:                 Human adenocarcinoma; ovary; ascites

 

Culture medium:                      McCoy's 5a medium with sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate + 10% fetal bovine serum. (Alternatively, DMEM:F-12 may be used)

Subculture routine:                 Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:2 to 1:3 is recommended

Morphology:                            epithelial

Karyotype:                              (P16) hypodiploid to hypotetraploid with dicentrics and large telocentric

AntigenExp:                            Blood Type B; Rh+

Isoenzymes:                           PGM3, 1; PGM1, 1-2; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0311

Tumorigenic:                           yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary

Depositor:                               Trempe

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Comments:                              Derived from a 64 years old caucasian femal

ATCC No.:                               HTB-77

 

 

Reference:     

Fogh, J. , ed., Human tumor cells in vitro. New York: Plenum Press; 1975:pp. 115-159. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Morimoto H et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. Morimoto H et al. Synergistic effect of tumor necrosis factor-alpha- and diphtheria toxin- ediated cytotoxicity in sensitive and resistant human ovarian tumor cell lines. J. Immunol. 147:2609-2616, 1991. Zhang X et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758,1997. Clinton GM et al. Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells. Proc. Natl. Acad. Sci. USA 93: 316-320, 1996. Zhang X and Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996.