Cell Line:                              SK-OV-3

Cell Description:                 Human adenocarcinoma; ovary; ascites


Culture medium:                      McCoy's 5a medium with sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate + 10% fetal bovine serum. (Alternatively, DMEM:F-12 may be used)

Subculture routine:                 Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:2 to 1:3 is recommended

Morphology:                            epithelial

Karyotype:                              (P16) hypodiploid to hypotetraploid with dicentrics and large telocentric

AntigenExp:                            Blood Type B; Rh+

Isoenzymes:                           PGM3, 1; PGM1, 1-2; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0311

Tumorigenic:                           yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary

Depositor:                               Trempe

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Comments:                              Derived from a 64 years old caucasian femal

ATCC No.:                               HTB-77




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