Cell Line:                              SK-N-LO

Cell Description:                 Human neuroblastoma

 

Culture medium:                      MEM (Eagle) with Earle«s BSS, sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate, 0,1mM non-essential amino acids + 10% FBS

Subculture routine:                 Remove medium, rinse with fresh 0,25% trypsin solution and let the culutre sit at room termperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subculture:                             every 6 to 8 days.

Split ratio:                                1:6 to 1:12 is recommended

Morphology:                            Epithelial

Growth Properties:                 Monolayer

Depositor:                               Buck

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

 

Biosafety Level:                     1

References:                           Cancer Res. 1988 Jun 1; 48(119: 2993-7; Anticancer Res. 1994 Jan-Feb; 14(1A):221-7; Melanoma Res. 1994 Apr; 4(2): 101-6; Anticancer Res. 1994 Sep-Oct; 14 (5A): 1903-6; Anticancer Res. 1997 Mar-Apr; 17(2A): 981-6; Anticancer Res. 1997 Jul-Aug; 17(4B): 3177-8.