Cell Line:                              SK-MEL-1

Cell Description:                 Human malignant melanoma (skin primary), metastasis to lymphatic system (thoracic duct)


Culture medium:                      MEM (Eagle) with Earle«s BSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 0,1 mM non-essential amino acids + 10% FBS

Subculture routine:                 Centrifugation and then resuspension. A concentration of 2 x 105 cells/ml is optimal.

                                               Incubate at 37¡C, 5% CO2

Morphology:                            Spherical

Karyotype:                              Chromosome Frequency Distribution 50 Cells: 2n=46.

                                               This is a hypertriploid human cell line. The modal chromosome number is 78 occurring at 36%, with polyploidy at 5.9%. The marker chromosomes del(2)(pl3), der(19)t(6;19)(pll;pl2/13), der(19)t(9;19)(pl3;qll), der(17)t(7;17)(qll;pl3), and 12 others are common to most cells. The first 2 markers listed abouve are generally single-copied and teh remaining 3 are paired (four other markers not listed abouve also paired). N12, N14 and N17 are mostly single-copied, wheras N15, N16, and N19 are 4-copied. The C and Y chromosomes have two copies each and the Y«s are further confirmed in QM-stained preparations.

Patient Data:                           Age 29; Sex male; Race Caucasian; Blood Type A+

Isoenzymes:                           PGM1, 1;PGM3, 1; ES-D, 1; GLO-1, 1-2; G6PD, B.

Sterility:                                   Tests for mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ATCC designation:                  HTB 67