Cell Line:                              SK-LMS-1

Cell Description:                 Human leiomyosarcoma; vulva


Culture medium:                      MEM (Eagle) in Earle«s BSS with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 0,1mM non-essential amino acids + 10% FBS

Subculture routine:                 Remove medium, add fresh 0,25% trypsin solution for 3 to 5 minutes, remove trypsin , add fresh medium, aspirate and dispens into new flasks. Subculture every 6 to 8 days

Split Ratio:                               1:2 to 1:5 is recommended

Morphology:                            Fibroblast

Karyotype:                              (P12) hypotriploid to hypertriploid (+A2, +A3, +C, +D, +E, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, breaks, secondary constrictions, minutes and large submetacentric markers.

AntigenExp:                            Blood Type 0; Rh+

Isoenzymes:                           Mw-2, 2; PGM3, 1-2; PGM1, 1-2;ES-D, 1; AK-1; GLO-1, 1-2; G6PD, B;

Phenotype frequency Prod.: 0.0027

Tumorigenic:                           yes, in nude mice; forms leiomyosarcoma

Depositor:                               Old

Comments:                              Established from 43-year-old female Caucasian

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ATCC designation:                  HTB 88

Reference:       Fogh, J., ed., Human tumor cells in vitro. New Yourk: Plenum Press; 1975:pp. 115-159. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. Koochekpour S et al. Met and hepatocyte growtz factro/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. Hu M et al. Purification and characterization of human lung firbrblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997