Cell Line:                              SK-BR-3

Cell Description:                 Human; pleural effusion (metastasis); adenocarcinoma

(colon primary)

 

Culture medium:                      McCoy's 5a medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Subculture routine:                 Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:2 to 1:4 is recommended

Morphology:                            Epithelial

Karyotype:                              (P9) hypertriploid to hypotetraploid (+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker

AntigenExp:                            Blood Type A; Rh+; HLA A11, Bw22(+/-), B40, B18

Isoenzymes:                           PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0044

Tumorigenic:                           yes, in nude mice; forms poorly differentiated adenocarcinoma

Depositor:                               Trempe

Comments:                              Established from 43-year-old female Caucasian

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ATCC designation:                  HTB-30

 

Reference:                            Fogh, J., ed., Human tumor cells in vitro. New York: Plenum

Press; 1975:pp. 115-159.Trempe GL. Human breast cancer in culture. Recent Results Cancer Res.: 33-341, 1976. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214,1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. Chavany C et al. p185erbB2 binds to GRP94 in vivo. J. Biol. Chem. 271: 4974-4977, 1996.