Cell Line:                              RPMI 1788

Cell Description:                 Human peripheral blood, B lymphocyte, EBNA positive

 

Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 20% FBS

Subculture routine:                 Maintain culutres between 3 Š 4 x 105 Cells/ml; Maximum cell density 1 Š 2 x 106 cells/ml.

Incubate at 5% CO2, 37”C

Morphology:                            Lymphoblast

Karyotype:                              2n = 46

Cell product:                           IgM (Lambda light chain)

Histocompatibility type:           A2, Aw33, B7, B14

Depositor:                               Obtained from ATCC

Comments:                              Derived from the peripheral blood of an apparently normal 33 year old Caucasian male. The cells secrete IgM (lambda light chain). Handle as a potential biohazard under at least containment level 2.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     2

ECACC number:                      85112106

ATCC designation:                  CCL 156

Reference:                             J. Nat. Cancer Inst. 1969; 43:1119