Cell Line:                              Reh

Cell Description:                 Human leukaemia, acute lymphocytic (non-T; non-B)


Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS

Subculture routine:                 Cultures can be maintained by addition or replacement

of medium. Start cultures at 2x 106 cells/ml and

maintain between 1 x 105 and 1 x 106  cells/ml.

Incubate at 5 % CO2, 37¡C

Morphology:                            Lymphoblast

Growth properties:                 Suspension

Anigen Exp:                            CD3 A (17%) B(17%) C(20%), CD4(15%),

CD10 (55%)

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Reference:                             Biochem. Biophys. Acta 516: 192-230, 1978

ATCC designation:                  CRL 8286