Cell Line:                              RD

Cell Description:                 Human embryo rhabdomyosarcoma, muscle


Culture medium:                      DMEM with 4,5 g/l glucose (hg) with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS

Subculture routine:                 Add fresh 0,25% trypsin and place at 37¡C for 3 to 5 minutes. Add fresh culture medium, aspirate and dispense into new culture vessels.

Split Ratio:                               1:2 is recommended

Morphology:                            spindle cells and large multinucleated cells

VirusSuscept:                        Poliovirus 1; vesicular stomatitis (Indi); herpes simplex; vaccinia

Reverse Transcriptase:         negative

Isoenzymes:                           G6PD, B

Products:                                myoglobin; myosin ATPase

Growth properties:                 monolayer

Depositor:                               McAllister

Comments:                             This line has recently been shown to be at least parental, if not identical, to TE 671 (ATCC: HTB 139)

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ECACC number:                     85111502

ATCC designation:                 CCL 136

Reference:      Vanderzant, C.; Splittstoesser, D.F., eds., Compendium of methods for the microbilogical examination of foods, 3rd ed.. Washington, DC: American Public Health Association; 19571-19577, 1996. McAllister RM et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. Chen TR et al. Cell identity resolved (leter; comment). Nature 340: 106, 1989. Nature 337: 311-312, 1989. Gershwin ME et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. J. Virol. 1: 326, 1967.