Cell Line:                              RC-134

Cell Description:                 Human kidney

 

Culture medium:                      McCoy«s 5a medium with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS

Subculture routine:                 Remove medium, rinse with fresh 0,025% trypsin

solution, remove trypsin and let the culture sit at room

temperature (or at 37¡C) until the cells detach (about

10 minutes). Add fresh medium, aspirate and dispense

into new flasks.

Subculture:                             every 4 to 6 days

Split ratio:                                1:3 to 1:6

Morphology:                            monolayer

Karyotype:                              2n = 46

Comments:                              Established from the kidney of a 58-year-old-male Caucasian, 1998

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Tumorigenic:                           no

Reference:                             not published