Cell Line:                              RC-131

Cell Description:                 Human kidney

 

Culture medium:                      McCoy`s 5a medium with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 90%; FBS, 10%

(Alternatively, DMEM / Ham`s F12 medium)

Subculture routine:                 Remove medium, rinse with fresh 0,025% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subculture:                             every 4 to 6 days

Split Ratio:                               1:3 to 1:6

Fluid Renewal:                        2 to 3 times weekly

Morphology:                            epithelial, cytokeratine 8, 18, 19 vimentin

Growth Properties:                 monolayer

Karyotype:                              2n = 46

Tumorgenetic:                         no

Comments:                              Established from a 60 year old caucasian male, 1998

Sterility:                                   Tests of mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Reference:                             not published