Cell Line:                              PC-3

Cell Description:                 Human adenocarcinoma; grade IV; prostate; from

metastatic site: bone

 

Culture medium:                      HamÕs F12K medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; FBS 10%

Subculture routine:                 Subcultures are prepared using CTC (Collagenase, 0,01% Trypsin, 0.02% and chick serum, 1%) at room temperature. Alternatively, subcultures can be prepared using a standard trypsin (0.25%) and EDTA (0.03%) dissociation protocol. The initial subculture is done 10 to 14 days after seeding thereafter

Split ratio:                                1:3 to 1:6

Morphology:                            Epithelial

Tumorgenic:                            yes, in nude mice

AntigenExp:                            HLA A1, A9

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negativ

Biosafety Level:                     1

Properties:                              monolayer; the cells form clusters in soft agar and can be adapted to suspension growth

Comments:                              The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.

ATCC designation:                  CRL 1435                                          

Reference:     

Kaighn ME et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62:183-184, 1993. Ohnuki Y et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534. 1980. Sheng S et al Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. Umekita Y et al. Human prostate tumor growth in athymic mice: inhibiton by androgens and stimultaion by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. Carter RE et al. Prostate-specific membrane antigen is a hydrolase with substrate and phramacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. Nupponen NN et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. Geiger T et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard cheomtherapeutic agents against various human tumors transplanted into nude mice. Anti-Cancer Drug Des. 13: 35-45, 1998. Su ZZ e al. Surface-epitope masking an expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family.  Proc Natl. Acad. Sci. US A 93: 7252-7257, 1996