Cell Line:                              PANC-1

Cell Description:                 human epithelioid carcinoma; pancreas; duct

 

Culture medium:                      DMEM with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS

Subculture routine:                 Remove medium, rinse with fresh 0.25% trypsin, 0.02% versene solution, withdraw and add 1 to 2 ml of trypsin, versene. Allow the culture to sit at room temperature or 37¡C until cells are dispensed, add fresh medium, aspirate and dispense into new flasks.

Split ratio:                                1:2 to 1:4

Morphology:                            Epithelial

Karyotype:                              modal number = 63; 3 marker chromosomes; 1 ring

Isoenzymes:                           G6PD, B

Doubling time:                         52

Growth properties:                 monolayer

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Depositor:                               M. Lieber

ECACC designation:               092802; ATCC No. CRL-1469

Comments:                              Growth is inhibited by 1 until/ml L-asparaginase. The cells will also grow  in soft agar.

Reference:     

 

Lieber M et al. Establishment of a continous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975; Wu MC et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978; Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001