Cell Line:                              PA-1

Cell Description:                 Human ovary teratocarcinoma

 

Culture medium:                      EMEM (EBSS) with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 1% NEAA + 10% FBS (heat inactivated)

Subculture routine:                 Split confluent cultures 1:3 to 1: 10 i.e. seeding at 1 x 104 to

3 x 104 cells/cm2  using 0,25% trypsin or  trypsin / EDTA; Incubate at 5% CO2, 37¡C

Morphology:                            Epithelial

Karyotype:                              2n = 46

Comments:                              Derived from ascitic fluid cells of a 12 year old Caucasian female with teratocarcinoma of the ovary. The cells produce tumours in nude mice.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ECACC number:                      90013101

ATCC designation:                  CRL 1572

Reference:                             In Vitro, (1974), 10, 382; J. Nat Cancer Inst, (1974), 52, 921; Int. J. Cancer, (1980), 25, 19