Cell Line:                              NAMALWA

Cell description:                 Human Burkitts lymphoma

 

Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate and 10% FBS

Subculture routine:                 Maintain cultures between 3 to 9 x 105 cells/ml;

A maximum density of 2 x 106 cells/ml is possible.

Incubate at 5% CO2, 37¡C

Morphology:                            Lymphoblast

Karyotype:                              2n = 46

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Depositor:                               Obtained from ATCC

Reference:                             Int J Cancer, (1972), 10, 44; Int J Cancer, (1973), 12, 396; Antimicro Agents Chemother, (1979), 15, 420

ECACC number:                      87060801

ATCC designation:                  CRL 1432