Zellinie

 

Cell name: Indian Muntjac

Cell description: muntjac, skin
Age/Stage: adult Gender: male
Morphology: fibroblast
Depositors: D.H. Wunsten
Virus Suscept: vesicular stomatitis (Indiana); herpes simplex; vaccinia Virus Resist: poliovirus 1
Reverse Transcriptase: negative
Passage submitted to the ATCC: unknown
Fluid Renewal: 2 times weekly
Sub Culturing: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 2 to 5 minutes, remove trypsin, EDTA and let flask sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Split Ratio: A ratio of 1:2 to 1:4 is recommended
Growth Properties: monolayer
Propagation: Medium: Ham's F10 medium, 80%; fetal bovine serum, 20%
Biosafety Level: 1

References:
Bolin SR et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994 PubMed: 95081240
Wurster DH and Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168: 1364-1366, 1970

ATCC Number: CCL-157

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