Cell Line: MRC-5
Cell Description: Human foetal lung
Culture medium: EMEM with EBSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate and 1% NEAA + 10% FBS
Incubate at 5% CO2, 37¡C
Karyotype: 2n = 46; diploid
Comments: Established from normal lung tissue of a 14 week old male foetus.
Mortality: The cells undergo between 60-70 population doublings before senescence.
Virus Susceptibility Is similar to WI-38
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
ECACC number: 84101801
ATCC designation: CCL 171
Reference: Nature, (1970), 227, 168