Cell Line: MOLT-4
Culture medium: RPMI 1640 with 4,5 g/L glucose, sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 10mM HEPES, 90%; FBS, 10%
AntigenExp: CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)
Products: High levels of terminal deoxynucleotidyl transferase (TdT) are produced
Comments: The cells do not produce immunoglobulin or Epstein-Barr virus. There is a G-> A mutation at codon 248 of the p53 gene. P53 is not expressed. The patient had received prior multidrug chemotherapy. MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC: CRL 1552).
ATCC designation: CRL 1582
Reference: Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993