Cell Line: MOLT-3
Cell Description: Human acute T lymphoblastic leukaemia
Culture medium: RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS
Incubate at 5% CO2, 37¡C
A maximum cell density of 1-2 x 106/ml is achieved.
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
Comments: Derived from the peripheral blood of a 19 year old male with acute lymphoblastic leukaemia whilst in relapse. A stable T-cell leukaemia line. The cells should be handled under laboratory containment level 2 coditions.
ECACC number: 90021901; ATCC No: CRL-1552
Reference: J. Nat. Cancer Inst. 1972; 49:891