Cell Line:                              MG-63

Cell Description:                 Human Osteosarcoma, bone


Culture medium:                      Minimum essential medium (Eagle) in Earle's BSS with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 1% non-essential amino acids, 90%; heat-inactivated fetal bovine serum, 10%

Subculture routine:                 Remove medium, add fresh 0.025% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:4 to 1:8 is recommended

Fluid Renewal:                        2 to 3 times weekly

Morphology:                            Fibroblast

Growth Properties:                 monolayer

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1                    

Depositor:                               Billiau  

Comments:                              Established from  a 14 year old caucasian male

Description:                            High levels of interferon production can be induced using polyinosinic-polycytidylic acid,cycloheximide and actinomycin D.

Receptors Expressed:           transforming growth factor beta (TGF beta, type I and type II)

Products:                                interferon

Reference:                             Billiau A et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. Takeuchi Y et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996.