Cell Line:                              MCF7

Cell Description:                 Human breast adenocarcinoma

 

Culture medium:                      EMEM (EBSS) with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 1% NEAA and 10% FBS

Subculture routine:                 Split confluent cultures 1:2 to 1: 6 i.e. seeding at 2 x 104 to 4 x 104 cells/cm2  using 0,25% trypsin or trypsin / EDTA;

Incubate at 5% CO2, 37¡C

Morphology:                            Epithelial

Biosafety Level:                     1

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

 

Karyotype:                              2n = 46; hypertriploid to hypotetraploid

 

Comments:                              Established from the pleural effusion from a 69 year old female Caucasian. The cells exhibit some of the features of differentiated mammary epithelium including oestradiol synthesis. The cells may carry B or C type virus and should be handled in category 2 (P2) containment.

ECACC number:                      86012803

ATCC designation:                  HTB-22

Reference:                             J. Nat. Cancer Inst, (1973), 51, 1409