Cell Description: Human carcinoma; prostate; from metastaic site: left supravicular lymh node
Culture Medium: RPMI 1640 medium with 4.5 g/L glucose, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate and 10 mM HEPES, 90%; FBS, 10%
Subculturing: Remove medium, rinse monolayer with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Split Ratio: A ratio of 1:3 to 1:6 is recommended
Fluid Renewal: twice weekly
Growth Properties: clusters; lightly adherent
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
Tumorigenic: yes, in nude mice
Karyotype: pseudodiploid male; seven marker chromosomes; modal number = 46; range = 33 to 91
Receptors Expressed: androgen; estrogen
Products: human prostatic acid phosphatase; prostate specific antigen
Comments: estabilshed from a 50-year-old caucasian male
References: Horoszewicz JS et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. Murphy, G.P., ed., Models for prostate cancer. 37, New York:Liss; 1980:pp. 115-132. Gibas Z et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. Boffa LC et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996.
Umekita Y et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996.
Hu SX et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997.