Cell Line:                              LNCaP

Cell Description:                 Human carcinoma; prostate; from metastaic site: left supravicular lymh node


Culture Medium:                      RPMI 1640 medium with 4.5 g/L glucose, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate and 10 mM HEPES, 90%; FBS, 10%

Subculturing:                          Remove medium, rinse monolayer with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:3 to 1:6 is recommended

Fluid Renewal:                        twice weekly

Morphology:                            epithelial

Growth Properties:                 clusters; lightly adherent

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Tumorigenic:                           yes, in nude mice

Karyotype:                              pseudodiploid male; seven marker chromosomes; modal number = 46; range = 33 to 91

Receptors Expressed:           androgen; estrogen

Products:                                human prostatic acid phosphatase; prostate specific antigen

Depositor:                               CLS

Comments:                              estabilshed from a 50-year-old caucasian male

References:                           Horoszewicz JS et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. Murphy, G.P., ed., Models for prostate cancer. 37, New York:Liss; 1980:pp. 115-132. Gibas Z et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. Boffa LC et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996.

Umekita Y et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996.

Hu SX et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997.