Cell Line:                              KATO-III

Cell Description:                 Human gastric carcinoma; stomach; pleural effusion; from metastatic site: supraclavicular and acillary lymph nodes and Douglas cul-de-sac

 

Culture medium:                      Iscove«s modified Dulbecco«s medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 20% FBS or RPMI1640 medium + 20% FBS

Subculture routine:                 Cultures can be maintained by addition or replacement of fresh medium, Adherent cells can be dislodged by scraping. Subculrture every 6 to 8 days.

Morphology:                            Spherical

Growth Properties:                 Suspension/monolayer

Karyotype:                              The stemline chromosome number is hypotetraploid with the 2S component occurring at 6,2%. Nine markers were common to most S metaphses, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.

Isoenzymes:                           PGM3, 1; PGM1, 1; ES-D, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0742

AntigenExp:                            Blood Type B; Rh+

Tumorigenic:                           Yes; in cheek pouches of anty thymocyte serum treated hamsters; not tumorigenic in nude mice.

Depositor:                               Sekiguchi

Comments:                              From a 55-year old oriantal male.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ATCC number:                        HTB-103

Reference:                             Sekiguchi M et al. Establishment of cultured cell lines derived from a human gastric carcinoma. Jpn. J. Exp. Med. 48: 61-68, 1978. Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. Rieder G et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997