Cell Line:                              Jurkat E6-1

Cell Description:                 Acute T cell leukemia, human


Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS

Comments:                              This clone of the Jurkat-FHCRC cell line produces large amounts of interleukin-2 (IL-2) is produced with phorbol esters and either lectins of monoclonal antibodies against the T3 T cell antigen receptor. No IL-2 is produced in the absence of stimuli, and both stimuli are required for activation and lymphokine production. The line was cloned from Jurkat-FHCRC cells obtained from K. Smith and have tested negative for mycoplasma. The cell can also be induced to produce human g interferon.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ATCC designation:                  TIB 152

Submitted by:                          A. Weiss, Howard Hughes Medical Institute, University of California, San Francisco, CA

Reference:                             J. Immunol. 133:123-128, 1984