Cell Line:                                JEG-3

Cell Description:                    Human choriocarcinoma; placenta

 

Culture medium:                      MEM (Eagle) in Earles«s BSS with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 1% non essential amino acids (NEAA), 10% fetal bovine serum.

Subculture routine:                  Remove medium, rinse with fresh 0,25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Morphology:                            Epithelial

Growth Properties:                   Monolayer

Split Ratio:                               1:4 to 1:6 is recommended

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Level:                                      1

Tumorigenic:                            yes, in nude mice; forms malignant tumor consistent with choriocarcinoma.

Isoenzymes:                            PGM3, 1-2; PGM1, 1; ES-D, 1; GLO-1, 1-2; G6PD, B

Products:                                 human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone; able to transform steroid precursors to estrone and estradiol.

Comments:                              This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates.

ATCC designation:                  HTB 36

Reference:      

Kohler PO and Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977 Acta Endocrinol. Suppl. 153: 137-153, 1971. Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein: Cancer Res. 57: 3562-3568, 1997. Foesler WJ et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996.