Cell Line:                              JAR

Cell Description:                 Human choriocarcinoma; placenta

 

Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS

Subculture routine:                 Remove medium, add fresh 0,025% trypsin, 0,02% EDTA in Earle«s BSS without Ca2+ and Mg2+  for 5 minutes, disperse the cells with a curved Pasteur pipette and centrifuge at 800 rpm for 3 minutes. Remove the trypsin, add fresh medium, resuspend the pellet and dispense into new flasks. Subculture every 6 to 8 days.

Split Ratio:                               1:4 to 1:6 is recommended

Morphology:                            Epithelial

Growth Properties:                 Monolayer

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Isoenzymes:                           G6PD, B; PGM1, 1-2; ES-D, 2; AK-1, 1; GLO-1, 1; Phenotype Frequency Product: 0.0002

Products:                                estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/ml after reculturing.

Comments:                              The JAR line was established by R. A. Pattillo and associates directly form a trophoblastic tumor of the placenta. Established from a 24-year-old caucasian female.

ATCC designation:                  HTB 144

Reference:                             In Vitro 6: 398, 1971