Cell Line:                              HuT 78

Cell Description:                 human embryo lymphoblast

 

Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS

Subculture routine:                 Maintain cultures between 3 x 105 to 9 x 105; 5% CO2, 37¡C

Morphology:                            Lymphoblast

 

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Depositor:                               DR. G. Farrar, PHLS CAMR, Porton Down, Salisbury

Comments:                              Derived from peripheral blood of patient with Sezary syndrome. Cells exhibit the features of a nature T-cell line with inducer/helper phenotype. Cells produce IL-2 and express IL-2-receptors alpha IL2RA / beta IL2RB / gamma IL2RG.

ATCC number:                        TIB-161

Reference:                             J. Exp. Med. 1981; 154:1403; J. Exp. Med. 1959; 109:271; J. Biol. Chem, 1959; 234:1042