Cell Line:                              HuH7

Cell Description:                 Human liver carcinoma (Hepatoma)

 

Culture medium:                      DMEM or RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90 %, FBS 10 %

 

Subculture routine:                 Split the cultures when the cells close to 70 Š 80 %. If the cells become too close, the cells will die.

So seeding at 1 x 104 to 2 x 104 cells/cm2 , using 0,25% trypsin or trypsin / EDTA; incubate at 5% CO2, 37”C

Split Ratio:                               1:4 to 1: 6

Morphology:                            epithelial

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Established by:                       Nakabayashi, H. & Sato, J.

References:                           Cancer Res., 45, 6379-6383, 1985. Cancer Res., 42, 3858-3866, 1982. Tiss. Cul. Res. Commun., 12, 221-227, 1993. Journal of Hepatology, 27, 677-687, 1997.