Cell Line:                              HT 1080

Cell Description:                 human fibrosarcoma (male, 35 years, Caucasian)

Culture medium:                      MEM Eagle with Earle`s BSS, sodium bicarbonate, 0,1 mM non essential amino acids, 2mM L-Glutamine and 1mM sodium pyruvate, 90%; heat-inactivated FBS, 10%

Subculture routine:                 Remove medium, add fresh 0,25% trypsin, 0,02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or 37¡C) until cells detach (approximately 10 min). Add fresh medium, aspirate and dispense into new flasks. Split confluent cultures 1:4 to 1: 8

Morphology:                            Epithelial

Virus Suscept:                       poliovirus 1; vesicular stomatitis (Indiana); RD114; feline leukemia virus (FeLV)

Tumorigenic:                           yes, in immunosuppressed mice

Oncogene:                              ras +

Transcriptase:                        negative

Isoenzymes:                           G6PD, B

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Comments:                              The cells contain an activated N-ras oncogene.

ATCC number:            CCL-121

References:

Chen TR et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351, 1983. Geiser AG et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. Rasheed S et al. Characterization of a newly derived human sarcoma cell line (HAT-1080). Cancer 33: 1027-1033,1974. Adams RA et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-, 1968. Proc Am Assic. Cancer Res. 8: 1, 1967. Hu M et al. Purification and characterisation of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibrinectin. Cancer Res. 57: 3577-3584,1997. Iida A et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J. Virol. 70: 6054-6059, 1996. Brennemann M et al. Stimulation of intracromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc. Natl. Acad. Sci USA 93: 3608-3612, 1996. Seiffert D. Hydrolysis of platelet vitronectin by calpain. J. Biol. Chem. 271: 11170-11176,1996. Hocking AM et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996