Cell Line:                              HL-60

Cell Description:                 Human leukemia, acute promyelocytic; blood, peripheral


Culture Medium:                      Iscove's modified Dulbecco's medium with sodium bicarbonate, 4 mM L-glutamine, 1,0 mM sodium pyruvate adjusted, 80%; fetal bovine serum, 20%

Subculturing:                          Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 x 105 viable cells/ml and subculture at 1 x 106 cells/ml.

Fluid Renewal:                        every 2 to 3 days

Morphology:                            lymphoblast

Growth Properties:                 suspesnsion

Oncogene:                              myc +

Receptors Expressed:           complement; Fc

Isoenzymes:                           G6PD, B; PGM1, 1; PGM3, 1; ES-D,1; Me-2, 1; AK-1, 1; GLO-1, 1

Products:                                tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid

Depositor:                               Gallagher

Description:                            HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc-oncogene expression.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Reverse Transcriptase:         negative

Comments:                              Established from a 36 year old Caucasian female


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