Cell Line:                              Hep G2

Cell Description:                 Human hepatocyte carcinoma

 

Culture medium:                      EMEM (EBSS) with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 1% NEAA + 10% FBS or Williams Medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, + 10% FBS

Subculture routine:                 Split confluent cultures 1:3 to 1: 6 i.e. seeding at 2 x 104 to 3 x 104 cells/cm2  using 0,25% trypsin or trypsin / EDTA; 5% CO2, 37¡C

Morphology:                            Epithelial

Karyotype:                              2n = 46; modal No 55

Cell Product:                           Prothrombin, antithrombin III, a-feotoprotein complement, C3 activator, fibrinogen

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Depositor:                               Prof. B. Knowles, Wistar Institute, Philadelphia

Comments:                              Established from 15-year-old male Caucasian

ECACC number:                      85011430

ATCC designation:                  HB 8065

Reference:                             Nature 1979; 282:615; Science 1980; 209:497