Cell Line:                              Hep 2

Cell Description:                 Human epidermoid carcinoma; larynx

 

Culture medium:                      MEM Eagle with Earle«s BSS, sodium bicarbonate, 2mM L-glutamine, 0,1mM non-essential amino acids and 1.0mM sodium pyruvate, 90%; FBS, 10% (optional DMEM)

Subculture routine:                 Remove medium, add fresh 0,25%trypsin, 0,02% EDTA solution, rinse and remove trypsin. Allow flask to sit at room temperature (or 37¡C) until cells detach, add fresh medium, aspirate and dispense into new falsks.

Split ratio:                                1:4 to 1:10

Morphology:                            epithelial

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Virus suscept:                        Polovirus 1, adenovirus 3, vesicular stomatitis

Isoenzymes:                           G6PD, A

Comments:                              The cells are positive for keratin by immunoperoxidase staining.

ATCC designation:                  CCL 23

Reference:                             Jamaluddin M et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70. 1554-1563, 1996