Cell Line:                              HEK 293

Cell Description:                 Human embryonal kidney

 

Culture medium:                      EMEM (EBSS) with sodium bicarbonate + 1% NEAA or DMEM, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS (heat inactivated)

Subculture routine:                 Split confluent cultures 1:2 to 1:3 i.e. seeding at 3 x 104 to 5 x 104 cells/cm2 using 0,25% trypsin or trypsin / EDTA; 5% CO2, 37¡C. Cells detach at room temperature and may take several days to retach.

Morphology:                            Epithelial

Karyotype:                              2n = 46

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Depositor:                               Obtained from ATCC

Comments:                              Derived from human embryonal kidney cells transformed with sheared human adenovirus type 5 (Ad5) DNA. The cells are sensitive to human adenoviruses and adenovirus DNA. They can be used to isolate transformation defective host-range mutants of Ad5 und for titrating human adenoviruses.

ECACC number:                      85120602

ATCC designation:                  CRL 1573

Reference:                             J Gen Virol, (1977), 36, 59; Virology, (1977), 77, 319