Cell Line: HEK 293
Cell Description: Human embryonal kidney
Culture medium: EMEM (EBSS) with sodium bicarbonate + 1% NEAA or DMEM, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS (heat inactivated)
Subculture routine: Split confluent cultures 1:2 to 1:3 i.e. seeding at 3 x 104 to 5 x 104 cells/cm2 using 0,25% trypsin or trypsin / EDTA; 5% CO2, 37¡C. Cells detach at room temperature and may take several days to retach.
Karyotype: 2n = 46
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
Depositor: Obtained from ATCC
Comments: Derived from human embryonal kidney cells transformed with sheared human adenovirus type 5 (Ad5) DNA. The cells are sensitive to human adenoviruses and adenovirus DNA. They can be used to isolate transformation defective host-range mutants of Ad5 und for titrating human adenoviruses.
ECACC number: 85120602
ATCC designation: CRL 1573
Reference: J Gen Virol, (1977), 36, 59; Virology, (1977), 77, 319