Cell Line:                              Gimen

Cell Description:                 neuroblastoma, human

Culture Medium:                      Minimum essential medium Eagle with Earle`s BSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate 0,1 mM non-essential amino acids, 90%; FBS, 10%

Subculture Routine:                Remove medium, rinse with fresh 0,25% trypsin soltion, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Split ratio:                                1:6 to 1:12 is recommended

Properties:                              Monolayer

Morphology:                            Epithelial

Depositor:                               CLS

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Reverences:                          Int-J-Dev-Neurosci. 1994 Aug; 12(5): 405-10. J-Neurosci-Fes. 1995 Apr 15; 40(6):707-15

Comments:                            

Based on our previous observations that neuroblastoma (NB) cells express fibroblast growth factor-2 (FGF-2; basic FGF) and respond to it (Janet T. et al. (submitted); Wewetzer K. et al. (1993) J. Neurosci. Res. 36, 209-215), we attempted to find to what extent selected cytokines (interleukin (IL)-1 beta and interferon gamma (IFN gamma)) may modulate FGF-mediated proliferative activity and differentiation. The NB cell lines IMR-32, SH-SY5Y, GIMEN and LAN-1 and colorimetric assays were used for the determination of cell numbers. IL-1 beta (and several other ILs, including IL-1 alpha, -2, -3, and 6) per se did not affect proliferation of any cell line studied. IFN gamma inhibited growth of GIMEN and LAN-1 cells, but was uneffective on IMR-32 and SH-SY5Y cells. FGF-2 was antimitogenic for GIMEN cells. IFN gamma reversed and IL-1 beta enhanced this antimitogenic effect of FGF-2. FGF-2 per se did not affect LAN-1 cells and did not modulate the growth inhibitory actions of IFN gamma on these cells. FGF-2 induced proliferaton of IMR-32 and SH-SY5Y cells. This effect was not modulated by IFN gamma or IL-1 beta. These results suggest a heterogeneous response pattern of human NB cell lines towards the cytokines studied and complex interactions of FGF-2, IL-1 beta and IFN gamma.