Cell Line:                              G-361

Cell Description:                 Human malignant melanoma, skin


Culture medium:                      McCoyÕs 5a with sodium bicarbonate, with 2mM L-glutamine 1.0mM sodium pyruvate + 10% FBS

Subculture routine:                 Split confluent cultures seeding at 2-4 x 104 cells/cm2 using 0,25 trypsin or trypsin / EDTA; 5% CO2, 37¡C, cells are slow in attaching

Split ratio:                                1:3 to 1:6 is recommended

Fluid renewal:                         2 to 3 times weekly

Morphology:                            epithelial

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Depositor:                               ECACC; ATCC No: CRL-1424

Comments:                              G-361 was established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 populations doublings.