Cell Line:                              EB 1

Cell Description:                 Burkitt lymphoma, upper macilla, human

 

Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS

Growth Properties:                 Suspension

Karyology:                              Chromosome Frequency Distribution 30 Cells: 2n = 46

 

                                               Cells:                           15        14        1

                                               Chromosomes:           46        47        48

 

The cell line is aneuploid human female, with chromosome counts in the near-diploid range. Normal chromosomes N8, N11, and N14 are monosomic, with the remainder of autosomes usually being paired. The X chromosome is most often trisomic. Four marker chromosomes are found. Two of these (markers M1 and M3) involve the reciprocal translocation between chromosomes N8 and N14 associated with most Burkitt«s lymphoma cell lines first described by L. Zech., et al., Int. J. Cancer 17: 47, 1976, with the further eloboration of the myc and Ig gene role by the P. Leder, R. Taub, and C. M. Croce reports (Science (Washington, DC) 222: 765, 1983; Proc. Natl. Acad. Sci. USA 79: 7824 and 7837, 1982.

 

Isoenzymes:                           PGM1, 1; ES D, !; GLO-1, 2; G6PD, B.

Phenotype Frequency:           0,1429

Sterility:                                   Tests for mycoplasma, bacteria and fungi were negative.

Biosafety Level:                     1

Species:                                 Confirmed as human by isoenzyme analysis.

Patient Data:                           Age 9; Sex Female; Race Negro.

Comments:     

 

The EB1 line was isolated in 1963 by M. A. Epstein and Y. M. Barr from biopsy fragments and cell clumps of the lymphoma. These were seeded to insulin bottles in Eagle«s basal medium with 10% human serum and fed by medium addition. The cells grew as free-floating single individuals or doublets with a mean doubling time of about 48 hours and were identified as altered lymphoblasts on basis of groth and uniform morphology (Lancet 1: 252-253, 1964; J. Natl. Cancer Inst. (Bethesda) 34: 231-240, 1965). Viral pariticles (Epstein-Barr virus) were noted through electron microscopic examination (M. Epstein, et al., J. Exp. Med. 121: 761-770, 1965). A culture at passage 174 eas obtained from G. Trempe and L. J. Old in 1973. Handle as potentially biohazardous material under at least Biosafety Level 2 containment.

 

ATCC designation:                  TIB 152