Zellinie

 

Cell name: CV-1

Cell description: Cercopithecus aethiops (African green monkey), kidney
Age/Stage: 141 days
Gender: male
Morphology: fibroblast
Depositors: F.C. Jensen
Virus Suscept: poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40,
Reverse Transcriptase: negative
Freeze Medium: Culture medium, 95%; DMSO,5%
Fluid Renewal: 2 times weekly
Sub Culturing: Remove old medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspi- rate and dispense into new flasks.
Split Ratio: A ratio of 1:2 to 1:3 is recommended
Growth Properties: monolayer
Descriptions: Suitable host for transfection, especially by SV40 vectors. Derived from the kidney of male adult African green monkey.
Applications: transfection host
Propagation: Medium: Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Biosafety Level: 1

References:
RF32572: Proc. Natl. Acad. Sci. USA 53: 53, 1964 RF36462: Kit S et al. Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid".
Virology 27: 453, 1965

ATCC Number: CCL-70

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