Zellinie

 

Cell name: COS-7

Organism: Cercopithecus aethiops (African green Monkey)
Tissue: kidney; SV40 transformed
Morphology: fibroblast
Virus Suscept: SV40 (lytic growth); SV40 ts A209 at 40C; SV40 mutants with deletions in the early region
Products: T antigen
Fluid Renewal: 2 to 3 times weekly
Sub Culturing: Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Split Ratio: A ratio of 1:4 to 1:8 is recommended
Bio Safety: Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Growth Properties: monolayer
Descriptions: Suitable for transfection by vectors requiring expression of SV40 T antigen. African green monkey kidney fibroblast-like cell line established from CV-1 cells (ATCC CCL 70) transformed by an origin-defective mutant of SV40 which codes for wild- type T antigen. This line contains T antigen, retainns complete permissiveness fo lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.

Propagation: Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Comments: This line was derived from the CV-1 cell line (ATCC CCL-70) by transformation with an origin defective mutant of SV40 which codes for wild-type T antigen.
Applications: transfection host

References:
Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182, 1981
Fernandez LM and Puett D. Lys583 in the third extracellular loop of the lutropin/choriogonadotropin receptor is critical for signaling. J. Biol. Chem. 271: 925-930, 1996

ATCC Number: CRL-1651
ECACC Number: 87021302

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