Cell Line:                              Chang Liver

Cell Description:                 Homo sapiens (human), liver


Culture medium:                      Minimum essential medium (Eagle) in Earle«s BSS

with sodium bicarbonate, 2 mM L-glutamine 1% non-essential amino acids and 1mM sodium pyruvate, 90%; FBS, 10%


Subculture routine:                 Remove medium, add fresh 0,025% trypsin, 0,02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or at 37¡C) until cells detach. Add fresh medium, aspirate and dispense into new flasks.

Split ratio:                                1:4 to 1:8

Morphology:                            Epithelial

Tumorigenic:                           yes, in Syrian hamsters

Isoenzymes:                           G6PD, A

Virus Susceptibility:                poliovirus 1, 2, 3; adenovirus 3; vesicular stomatitis


Comments:                              Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. The cells are positive for kreatin by immunoperoxidase staining.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Reference:                             Proc. Soc. Exp. Biol. Med. 87: 440, 1954. RF36404: Natl. Cancer Inst. Monogr. 7: 249, 1962. Benn J et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996.