Cell Line: Capan-2
Cell Description: Human adenocarcinoma, pancreas
Culture medium: Mc Coy«s 5a medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS
In Vitro Cytopathology: (P8) Adenocarcinoma
Nude mouse: Produces well-differentiated adenocarcinoma consistent with pancreatic carcinoma
Description: The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
Comments: J. Fogh isolated this line in 1975 and characterized the progeny as indicated beloww. Patient Data: Age-56, male, Race-Caucasian; Blood Type-B+.
Species: Confirmed as human by isoenzyme analysis.
Isoenzymes: Me-2, 2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0004
Blood Group Antien: Confirmed as B on cultured Colls
Sterility: Tests for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
References: Dahiya R et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993.
ATCC designation: HTB 80