Caco-2/Medium: EMEM / Zoom: 200x

 

 

Cell Line:                              Caco-2

Cell Description:                 Human adenocarcinoma; colorectal; colon

 

Culture medium:                      Minimum essential medium Eagle with Earle's BSS, sodium bicarbonate, 2 mM L-glutamine, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate 80%; fetal bovine serum 20%

Subculture routine:                 Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Split Ratio:                               A ratio of 1:2 to 1:3 is recommended

Fluid Renewal:                        2 to 3 times weekly

Morphology:                            epithelial

Growth Properties:                 monolayer

Depositor:                               Fogh

Comments:                              Derived from a 72 year old caucasian male.

Virus Resistance:                   human immunodeficiency virus (HIV, LAV)

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Tumorigenic:                           yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II)

Karyotype:                              (P14), hypertetraploid

Antigen Expression:               Blood Type O; Rh+

Receptors Expressed:           heat stable enterotoxin (Sta, E. coli); epidermal growth factor (EGF), and expresses retinoic acid binding protein I, retinol binding protein II and are keratin positive

Isoenzymes:                           Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0187

Reference:     

 

Didier ES et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. Jumarie C and Malo C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Adachi A et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. Cohen MB et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. Basson MD et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. Heinen CD et al. Microsatellite instability in colorectal adenocarcinoma cell lines that have full-length adenomatous polyposis coli protein. Cancer Res. 55: 4797-4799, 1995. Gilbert T and Rodriguez-Boulan E. Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100 ( Pt 3: 451-458, 1991.Rigothier MC et al. A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study. Infect. Immun. 59: 4142-4146, 1991 . Levin MS. Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276, 1993. Trotter PJ and Storch J. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. White LJ et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. Baier LJ et al. A polymorphism in the human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells. J. Biol. Chem. 271: 10892-10896, 1996. Kutchera W et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. Grindstaff KK et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996