Caco-2/Medium: EMEM / Zoom: 200x



Cell Line:                              Caco-2

Cell Description:                 Human adenocarcinoma; colorectal; colon


Culture medium:                      Minimum essential medium Eagle with Earle's BSS, sodium bicarbonate, 2 mM L-glutamine, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate 80%; fetal bovine serum 20%

Subculture routine:                 Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Split Ratio:                               A ratio of 1:2 to 1:3 is recommended

Fluid Renewal:                        2 to 3 times weekly

Morphology:                            epithelial

Growth Properties:                 monolayer

Depositor:                               Fogh

Comments:                              Derived from a 72 year old caucasian male.

Virus Resistance:                   human immunodeficiency virus (HIV, LAV)

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Tumorigenic:                           yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II)

Karyotype:                              (P14), hypertetraploid

Antigen Expression:               Blood Type O; Rh+

Receptors Expressed:           heat stable enterotoxin (Sta, E. coli); epidermal growth factor (EGF), and expresses retinoic acid binding protein I, retinol binding protein II and are keratin positive

Isoenzymes:                           Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0187



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