Cell Description: Human mammary gland
Culture medium: RPMI 1640 medium with 4,5 g/l glucose, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 10 mM HEPES, 1,2 mM oxaloacetic acid, 0,02 mg/ml bovine insulin, 10 % NCTC 135 medium and 10 % fetal bovine serum.
Alternatively, DMEM:Ham«s F12 (1:1 mixture) supplemented with 2 mM L-glutamine, 1,0 mM sodium pyruvate and 10 % fetal bovine serum may be used.
Growth Properties: Monolayer; the cells form compact, multilayered colonies; growing slowly and rarely becoming confluent.
Description: The BT-474 line was isolated by E. Lasfargues and W. G. Coutinho from a solid, invasive ductal carcinoma of the breast.
Tumorigenic: yes, in nude mide
Karyotype: mode = 55; range = 50 to 112; bimodal shift 58 Š 59 and 100 in later passages with 3 marker chromosomes
Isoenzymes: Me-2, 0; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 1; Phenotype Frequency Product: 0.0426
Virus Susceptibility: mouse mammary tumor virus (RIII-MuMTV)
Comments: Derived from a 60 years old caucasian female.
Biosafety Level: 1
Lasfargues EY et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. Lasfargues EY et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. Kumar R, Shepard HM and Mendelsohn J. Regulation of phosphorylation of the c-erbB-2/HER2 gene product by a monoclonal antibody and serum growth factor(s) in human mammary carcinoma cells. Mol Cell Biol 11 (2): 979-986, 1991.