Cell Line:                              BeWo

Cell Description:                 Human placenta choriocarcinoma


Culture medium:                      Ham«s F12K medium with sodium bicarbonate, 2mM L-glutamine and 1,0mM sodium pyruvate, 90%; FBS 10%. Daily medium renewals are required to prevent glucose exhaustion.

Subculture routine:                 seeding at 4x104 cells / cm2; Fluid renewal 3 to 4 times weekly. Split confluent cultures 1:3, using trypsin/EDTA: Remove medium, add fresh 0,0025% trypsin, 0,03% EDTA for several minutes, remove trypsin and let culture sit at  37¡C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.

Morphology:                            epithelial, monolayer.

Describtion:                            The cells are positive for keratin by immunoperoxidase staining

Karyology:                              2n = 46

Sterility:                                   Tests for mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Isoenzymes:                           G6PD, B

Reverse transcripase:           negative

Virus susceptibility:                poliovirus 3; vesicular stomatitis (Indiana)

Products:                                hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin

ECACC number:                 86082803

ATCC designation:             CCL 98

Reference:                           Cancer Research, (1968), 28, 1231; Science, (1968), 159, 1467; Proc Soc Exp Biol Med, (1959), 102, 77