Cell Line:                              AsPC-1

Cell Description:                 Human metastatic pancreatic adenocarcinoma

 

Culture medium:                      RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 80%; FBS, 20%

 

Subculture routine:                 Split confluent cultures 1:3 to 1: 6 using 0,25% trypsin

 or trypsin / EDTA;

 

Comments:                              This pancreatic line was derived from ascites of a 62-year old female Caucasian. It was re-established from a tumoral xenograft in a nude mouse which expressed prototypic characteristics of the primary pancreatic adenocarcinoma.

 

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

ATCC designation:                  CRL 1682

Reference:                             In Vitro 18: 24-34, 1982; J. Natl. Cancer Inst. (Bethesdea) 67: 563-569, 1981; Tumor biol. 6: 89-98, 1985; Clin. Lab. Med. 2: 567-578, 1982